Reconstitution of the renal brush-border membrane sodium/phosphate co-transporter
نویسندگان
چکیده
منابع مشابه
Inhibition of the sodium-dependent uridine co-transporter in bovine renal brush-border membrane vesicles by group-specific reagents
T h e reabsorption of amino acids, glucose and organic anions from the renal filtrate into epithelial cells is coupled to Na' transport down its concentration gradient via specific cotransporters present in the brush-border membrane. Recently, Na + -dependent nucleoside transport has been demonstrated in the proximal tubule of rat kidney 11, 21. In our laboratory, a Na + -dependent uridine tran...
متن کاملKinetic evidence for a common transporter for glycylsarcosine and phenylalanylprolylalanine in renal brush-border membrane vesicles.
We investigated the interaction between glycylsarcosine (Gly-Sar), a neutral dipeptide, and phenylalanylprolylalanine (Phe-Pro-Ala), a neutral tripeptide, for transport into renal brush-border membrane vesicles isolated from Japanese F344 rats. This rat strain is genetically deficient in dipeptidylpeptidase IV. Owing to the absence of this enzyme, Phe-Pro-Ala was found to be completely resistan...
متن کاملThe renal-specific transporter mediates facilitative transport of organic anions at the brush border membrane of mouse renal tubules.
The renal secretion of organic anions across the proximal tubules is achieved by a coordination of uptake and efflux transporters. This study reports the expression, localization, and functional properties of mouse renal-specific transporter (RST). Mouse RST mRNA is predominantly expressed in the kidney and localized on the brush border membrane of mouse kidney proximal tubules. Mouse RST-expre...
متن کاملbrush-border membrane vesicles
The characteristics of hypoxanthine transport were examined in purified brush-border membrane vesicles isolated from guineapig kidney. Hypoxanthine uptake in the vesicles was specifically stimulated by both Na+ and an inside-negative potential, resulting in a transient accumulation of intravesicular hypoxanthine. Na+-dependent hypoxanthine influx was saturable (apparent Km 4.4 + 2.1 ,uM, Vmax 1...
متن کاملDexamethasone modulates rat renal brush border membrane phosphate transporter mRNA and protein abundance and glycosphingolipid composition.
Glucocorticoids are important regulators of renal phosphate transport. This study investigates the role of alterations in renal brush border membrane (BBM) sodium gradient-dependent phosphate transport (Na-Pi cotransporter) mRNA and protein abundance in the dexamethasone induced inhibition of Na-Pi cotransport in the rat. Dexamethasone administration for 4 d caused a 1.5-fold increase in the Vm...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1991
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2780543